Method of selection:
pJB8 and pHC79 ampicillin
Lorist kanamycin
pYAC4 yeast ura-medium
G pfosl chloramphenicol
Note that in most cases Lorist clones are the easier cosmids to grow;
because, unlike ampicillin, kanamycin is not destroyed by the cells.
Clone name: Vector: A Lambda 2001 Sau3AI partial ZL Lambda 2001 Sau3AI partial probed YSL Lambda 2001 YAC subclones, Sau3AI partial B pJB8 serial Sau3AI partial C pJB8 gridded in microtiter plates Sau3AI partial D pJB8 gridded on filters Sau3AI partial E pJB8 EcoK minus Sau3AI partial R pJB8 RI partial M pJB8 MboI partial ZC pJB8 probed K Lorist B Sau3AI partial T Lorist 2 Sau3AI partial W Lorist 6 NotI plus Sau3AI partial F Lorist 6 Sau3AI partial ZK 15 - 56 Lorist 6 Sau3AI partial probed 57 - 130 Lorist 2 Sau3AI partial probed 131 - 164 Lorist 6 Sau3AI partial probed 165 - 177 Lorist 2 Sau3AI partial probed 178 - 192 Lorist 6 Sau3AI partial probed 193 - 344 Lorist 2 Sau3AI partial probed 345 - 354 Lorist 6 Sau3AI partial probed 355 - 514 Lorist 2 Sau3AI partial probed 515 - 552 Lorist 6 Sau3AI partial probed 553 - 596 Lorist 2 Sau3AI partial probed 597 - 616 Lorist ? Sau3AI partial probed 617 - 626 Lorist 2 Sau3AI partial probed 627 - 692 Lorist 6 Sau3AI partial probed 693 - 756 Lorist 2 Sau3AI partial probed 757 onwards Lorist 6 Sau3AI partial probed Y pYAC4 RI partial Yeast: PEL pel61 SmaI 9... pphc79 SmaI Additional information ---------- ----------- Name: Vector: Cell: Selection: Cloning site: Organism: H_DJ pcypac2 DH10B kan Sau3A in BamH1 (GATC) Human(RPCI1,3-5) G pfos1 XL1 chlor Sau3A in BamH1 (GATC) C. briggsae H pfos1 XL1 chlor Sau3A in BamH1 (GATC) C. elegans MM lorist6 XL1 kan Sau3A in BamH1 (GATC) C. briggsae H_GS pbelo DH10B chlor HindIII (AAGCTT) Human H_RG pbelo DH10B chlor HindIII (AAGCTT) Human H_LUCA pwe15 ? kan EcoRI (GAATTC) Human (III) H_P1 p1 ? kan Sau3A in BamH1 (GATC) Human (III) H_U lawrist16 ? kan Sau3A in BamH1 (GATC) Human (X) H_LL lawrist16 ? kan Sau3A in BamH1 (GATC) Human (7) A_TM pbelo ? chlor HindIII (AAGCTT) Arabidopsis A_IG pbelo ? kan EcoRI (GAATTC) Arabidopsis H_NH pBACe3.6 DH10B chlor EcoRI (GAATTC) human (RPCI11) H_DA pPAC4 DH10B kan MboI in BamHI (GATC) human (RPCI6) CB pBAC3.6 DH10B chlor EcoRI (GAATTC) C. briggsae